Coding

Part:BBa_K3126005

Designed by: Qianxin Li   Group: iGEM19_HBUT-China   (2019-10-15)
Revision as of 07:00, 20 October 2019 by Lqs991122 (Talk | contribs)


hexa-His

This is a surface display system on the the yeast surface using the α-agglutinin method, it can display hexa-his on the yeast cell wall.


Usage and Biology

We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of Saccharomyces cerevisiae cell wall.

<img src="Theory_of_Hexa-his.png" width="80%"> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 343
    Illegal AgeI site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None