Composite
Part:BBa_K3196016
Designed by: Hao Qiu Group: iGEM19_HUST-China (2019-10-16)
Revision as of 05:46, 20 October 2019 by Zyz2018hust (Talk | contribs)
AOX1-Kozak-FLO10-pelA-His tag-AOX1 Terminator
The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.
Characterization
This is a four section for degrade and transfer pectin part.
DNA Gel Electrophoretic
After we link SUC2 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |