Part:BBa_K2922027

Colicin-N coding region

Summary

As a kind of bacteriocin secreted by E.coli, colicin can kill other related bacteria that can’t secret specific immunity proteins. Similar to Colicin-E1, Colicin-N is also a type of pore-forming colicin, encoded by gene cna. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.[1] (Fig.1)

Fig.1 Colicin-N can kill other bacteria.

Identification

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-1168bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] E. Cascales et al., Colicin biology. Microbiol Mol Biol Rev 71, 158-229 (2007).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 931
  
• 1000
  COMPATIBLE WITH RFC[1000]