Part:BBa_K3165043
CcaS-ECFP + Ho1-PcyA-CcaR
This part consists of all the components of the CcaS-CcaR optogenetic system with a GFP derived fluorescent protein, ECFP downstream of the pCpcG2 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2749
Illegal NheI site found at 2772
Illegal NheI site found at 4500
Illegal NheI site found at 4523 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
CcaSR is a green-light activated two-component signal transduction system (TCS) that controls the expression of phycobilisome linker protein CpcG2 in Synechocystis sp. PCC6803 complementary chromatic adaptation pathway. The naturally occurring CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. Upon activation with green light, the CcaS is autophosphorylated to a red-absorbing conformation that undergoes phosphotransfer to the response regulator CcaR leading to the activation of the PcpcG2 promoter initiating transcription of the gene of interest. In this gene construct, ECFP (Engineered Cyan Fluorescent Protein) is used as the gene of interest which acts as a fluorescent marker to quantify the activity of the photo-sensitive system. This part is used as a dynamically controlled light-mediated protein expression system.
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