Part:BBa_K2957043
CMV-2xGal4 (Gal4 repressible promoter)
Contents: CMV-2xGal4 (Gal4 repressible promoter)
What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
What does it do?: It codes for a Gal4 repressible promoter, which is involved in the synnotch system. It was important for our project in particular because of its involvement in the synNotch system and is known to be repressible, which we wanted to incorporate into our swarming mechanism.
How to use it?: You can use this part for Type IIS Cloning. It is usually found in a plasmid with BsaI cut sites that allows for this. With other Basic parts like pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the promoter part alone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 92
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 92
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 92
- 1000COMPATIBLE WITH RFC[1000]
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