Coding

Part:BBa_K3264018

Designed by: Xinyou Chang   Group: iGEM19_GreatBay_SZ   (2019-10-19)
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sfGFP-NT-2Rep-CT

A wide range of biomedical application of spider silk is occurring due to its features of high stress and strain tolerance. The current approach is to produce recombinant spidroins (silk proteins) from other chassis and spin them into silk. This year we are willing to improve the original spidroin NT-2Rep-CT that we have constructed and solve the problem of silk dyeing and color fastness on color silk, instead of the pure NT-2Rep-CT spidroin made silk. NT-2Rep-CT, the part we synthesized to for artificial silk spinning, is the common architecture in two main types of spider silk proteins: major ambulate spidorins(MaSps) and minor ampullate spidorins (MiSps): NT is a non-repetitive N-terminal domain, as well as CT, the C-terminal domain. In between them is an extensive repetitive region-Rep. In the storage of spider silk protein, NT is a monomeric and highly soluble region in neutral pH, and it provides the solubility of the entire spidorin. NT can form stable dimers when pH decreases in the spinning duct. On the other side during the decrease of pH, the CT gets disrupted and unfold to turn in the beta-sheet amyloid-like fibrils. It is hypothesized that structural change of CT precipitate the change of the repetitive region to beta-sheet conformation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 40


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