Composite

Part:BBa_K2957035

Designed by: Melody Wu   Group: iGEM19_MIT   (2019-10-19)
Revision as of 02:08, 20 October 2019 by Mwu (Talk | contribs)

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TRE-tight, mKate

Contents: TRE, mKate2, SynthpA, with Amp resistance

What is it?: A Composite part; it is a genetic circuit with TRE-tight promoter and mKate2 reporter gene.

What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid.

If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
    Illegal AgeI site found at 1057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 784
    Illegal BsaI.rc site found at 973


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