DNA

Part:BBa_K2985160

Designed by: Diqing Sheng   Group: iGEM19_Jiangnan-China   (2019-10-15)
Revision as of 01:01, 20 October 2019 by JNUsdq (Talk | contribs)


P43-liaH->Lipopeptide tolerance gene

Enhanced tolerant genes for lipopeptides, so that the liaH gene will not be expressed only when the surfactin reaches a certain concentration, but will begin to synthesize a specific tolerance protein during the growth process, thus protecting Bacterial cells. The liaH gene cluster is mainly involved in the coding of the protein Lia protein, which can protect the integrity of the cell membrane. At the same time, liaH exists in the large gene cluster of lia, and the two-component system liaRS can effectively resist the damage of cells such as surfactin. It can enhance the tolerance of bacteria to lipopeptide antibiotics.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 149
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 149
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None