Generator

Part:BBa_K3165038

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)
Revision as of 01:00, 20 October 2019 by Mohitdas (Talk | contribs)


Gp2 under PcpcG2 Expression

This part places the bacteriostatic growth-inhibitory protein, Gp2 under the inducible promoter: pCpcG2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for a bacteriostatic growth-inhibitory protein, Gp2 under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
Upon light stimulation, the production of Gp2 is triggered leading to a bacteriostatic effect. On withdrawal of the light stimuli, the production of Gp2 is hindered and owing to its low half-life, the Gp2 already present in the cells is also degraded.
This part is used in association with the CcaS-CcaR system as a light-induced growth regulation system.


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Categories
Parameters
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