Composite

Part:BBa_K2957015

Designed by: Melody Wu   Group: iGEM19_MIT   (2019-10-19)
Revision as of 00:04, 20 October 2019 by Registry (Talk | contribs)

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CMV, CCL5

Contents: CMV, CCL5, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None