Translational_Unit

Part:BBa_K3165033

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)
Revision as of 23:44, 19 October 2019 by Mohitdas (Talk | contribs)


RBS + ECFP

The codon optimised engineered cyan fluorescent protein (ECFP) is placed along with a ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker. ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.

Usage

This part is used as a reporter unit under any suitable promoter. Under a photo-sensitive promoter system, this part can be used to characterise the functioning of the inducible system based on fluorescence measurements.


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Categories
Parameters
None