Part:BBa_K2957008

CMV, IL-8 with NeonGreen Tag (C-terminal)

Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1802
  Illegal SpeI site found at 92
  Illegal PstI site found at 1245
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 92
  Illegal PstI site found at 1245
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1019
  Illegal BamHI site found at 1261
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1802
  Illegal SpeI site found at 92
  Illegal PstI site found at 1245
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1802
  Illegal SpeI site found at 92
  Illegal PstI site found at 1245
  Illegal AgeI site found at 1808
  
• 1000
  COMPATIBLE WITH RFC[1000]