Part:BBa_K2957003
hEF1a, IL-8 (CXCL-8)
Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 567
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 485
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 567
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 567
Illegal AgeI site found at 81
Illegal AgeI site found at 573 - 1000COMPATIBLE WITH RFC[1000]
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