Part:BBa_K2973011:Design

Toehold 13 β-Lactamase Geobacillus kaustophilus

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This toehold was designed in order to detect the 16S rRNA of the hyperthermophile Geobacillus kaustophilus. The design was made with the NUPACK software http://www.nupack.org

Source

Geobacillus kaustophilus strain BGSC 90A1 16S ribosomal RNA, complete sequence

NCBI Reference Sequence: NR_115285.2

References

Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.

Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.

Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.

J. N. Zadeh, C. D. Steenberg, J. S. Bois, B. R. Wolfe, M. B. Pierce, A. R. Khan, R. M. Dirks, N. A. Pierce. NUPACK: analysis and design of nucleic acid systems. J Comput Chem, 32:170–173, 2011.

http://www.nupack.org/