Terminator

Part:BBa_K3192026:Design

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-14)
Revision as of 21:10, 19 October 2019 by Simonneguenette (Talk | contribs)


Double terminator designed for golden gate assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 136


Design Notes

This terminator is a redesign of the double terminator BBa_B0015, a very well characterized iGEM terminator. BasI restriction sites were added to each terminator end to increase the ease of requesting this part for the intended use of golden gate assembly.

The 2019 Virginia iGEM team utilized Golden Gate Assembly for their project, and are aware it is a popular assembly method for many other iGEM teams. Through the addition of restriction sites compatible for Golden Gate, Virginia IGEM hoped to increase the ease of designing constructs for Golden Gate. Team planning on using this part and assembling using Golden Gate can now do so with more ease. Please see the design page for additional design notes on how to further optimize this BioBrick for Golden Gate Assembly.


Source

Escherichia coli

References