Coding
bleR+scp

Part:BBa_K2984090

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)
Revision as of 21:09, 19 October 2019 by Registry (Talk | contribs)

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bleR+scp - Bleomycin resistance with self cleaving peptide

This part is composed of a bleomycin resistance gene fused to a self cleaving peptide.

The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every two bleomycin molecules, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:2 to the antibiotic (Hayes et al., 1990). By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme (Lumbreras et al. 1998).

The self cleaving peptide is designed to cleave after translation of the bleomycin resistance and a desired protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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