Terminator

Part:BBa_K3192026:Design

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-14)
Revision as of 20:49, 19 October 2019 by Simonneguenette (Talk | contribs) (→‎Design Notes)


Double terminator designed for golden gate assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 136


Design Notes

The sequence ggtctc is used for recognition of the BsaI restriction enzyme used for Golden Gate assembly. When cloning multiple inserts into a vector using Golden Gate assembly, it is recommended to add a 5 base pair recognition sequence following the restriction site, before you insert that is complementary to the adjacent insert of interest to increase the specificity of the reaction.

Source

Escherichia coli

References