Coding

Part:BBa_K2929000

Designed by: James Hammond   Group: iGEM19_St_Andrews   (2019-10-10)
Revision as of 19:57, 19 October 2019 by Zsoltsemperger (Talk | contribs)


Human IgG4 CH3 Domain

Codes for the CH3 Domain of the Human IgG4 Antibody. PDB ID: 4B53. Codons optimised for Escherichia coli. 5' NcoI and 3' BamHI cut-sites.

Should be noted that the part has an unintentional mutation C425F.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//proteindomain
Parameters
protein