Regulatory

Part:BBa_K3179003:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 19:26, 19 October 2019 by Bigxushen (Talk | contribs) (→‎Source)


miR592-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part, four repeating sequences complementary to miRNA592 on an mRNA, is a 4X miRNA target designed to target miRNA592. When miRNA592 reaches a certain concentration in the cell, it would combine with miR592-target and suppress the translation of mRNA. According to our design, miR592-target is designed to control the translation of mRNA with response parts. We integrate miR592-target with L7Ae so that L7Ae cannot be translated with miRNA592 existing in the cell.

Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References