Regulatory

Part:BBa_K3179002:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 19:23, 19 October 2019 by Bigxushen (Talk | contribs) (→‎Design Notes)


miR 885-5p-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to control the translation of mRNA with response parts. We integrate miR885-5p-target with 2kt-E1A and miR663b-target so that E1A cannot be translated with miRNA885-5p existing in the cell.

Source

miR 885-5p-target-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.

References