Composite
Part:BBa_K3081022
Designed by: Xiaoying Qiao Group: iGEM19_Peking (2019-10-19)
pBAD-dCas9-J23119-NT1
This composite part is the principal design of the inducible CRISPRi systemBBa_K3081024, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. So it needs to cotransform with a plasmid coding mRFP.Fluorescence is decreased as the concentration of arabinose increases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1470
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |