Coding
PETase

Part:BBa_K2984069

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)
Revision as of 18:33, 19 October 2019 by Chlamy Dima (Talk | contribs)


PETase CDS; Enzyme that catalyzes the first degradation step of PET

The PETase is an enzyme that was discovered in the bacterium Ideonella sakaiensis and catalyzes the hydrolysis of PET to MHET (Yoshida et al. 2016). This enzyme has caught the attention of many research groups around the world for its potential to be used for biotechnological applications regarding PET degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 861
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 400
    Illegal PstI site found at 861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 861
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 861
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B3-B3 MoClo overhangs can be found here.

This part with B2-B4 MoClo overhangs can be found here.



References

  1. Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199.
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Categories
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