Coding
PETaseB3B4

Part:BBa_K2984011:Design

Designed by: Alejandro Gaston Castro   Group: iGEM19_Humboldt_Berlin   (2019-10-13)
Revision as of 18:31, 19 October 2019 by Chlamy Dima (Talk | contribs) (→‎References)


PETase CDS; Enzyme that catalyzes the first degradation step of PET B3-B4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 879
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal PstI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 879
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199.

References

   Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199.