Composite

Part:BBa_K3081057

Designed by: Song-Yuan Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 17:48, 19 October 2019 by Xiaoying (Talk | contribs)

dCas9 J23119 M+ with the thermo-sensitive transcription factor TCI42

dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement ofBBa_K3081040, which we add a degradation signal peptide ssrA to the dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 753
    Illegal NheI site found at 776
    Illegal NheI site found at 5286
    Illegal NheI site found at 5309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None