Composite

Part:BBa_K3081003

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 16:27, 19 October 2019 by Xiaoying (Talk | contribs)


pBAD-dCas9-linker-sfGFP-ssrA

A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of dCas9-linker-sfGFP(No part name specified with partinfo tag.


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