Regulatory
hucO

Part:BBa_K3007000

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-14)
Revision as of 15:59, 19 October 2019 by Leaves (Talk | contribs)

HucR binding site

It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid.

Contribution: WHU-China 2019

Group: WHU-China
Authors: Jiongyi He
Summary: We confirmed the function of sfGFP(BBa_I746916) and determined the relationship between sfGFP protein concentration and fluorescence intensity.
Documentation:
SfGFP-WHU.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None