Coding

Part:BBa_J70024:Design

Designed by: Tom Knight   Group: Knight Lab   (2008-10-20)
Revision as of 18:39, 20 October 2008 by Tk (Talk | contribs) (→‎Design Notes)

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TetM gene, recoded compromise E. coli & Mesoplasma florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species.

GTTTCTTCGAATTCGCGGCCGCTtctagatgaaaatcataaatatcgg,J00024-F

GTTTCTTCCTGCAGCGGCCGCTactagtattattatgtgattttgttg,J00024-R

Source

Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. Genbank P09757.

References

Sanchez-Pescador,R., Brown,J.T., Roberts,M. and Urdea,M.S. The nucleotide sequence of the tetracycline resistance determinant tetM from Ureaplasma urealyticum Nucleic Acids Res. 16 (3), 1216-1217 (1988) PMID: 3344217