Regulatory

Part:BBa_J70025:Design

Designed by: Tom Knight   Group: Knight Lab   (2008-10-20)
Revision as of 18:38, 20 October 2008 by Tk (Talk | contribs) (→‎Design Notes)

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Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Alignment of the promoter with the coding region, preservation of the RBS sites.

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGggccagtctacatgtactc,J00025-F

GTTTCTTCCTGCAGCGGCCGCTACTAGTAttcctccattcaaaaacccaa,J00025-R

Source

pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid.

References

Su YA, He P, Clewell DB. Characterization of the tet(M) determinant of Tn916: evidence for regulation by transcription attenuation. Antimicrob Agents Chemother. 1992 Apr;36(4):769-78. PMID: 1323953