Composite

Part:BBa_K3196021

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 15:10, 19 October 2019 by Orange-Huo (Talk | contribs)


AOX1-Kozak-FLO10-VP-His tag-AOX1 Terminator

FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. T--HUST--China--2019-FLO10VP

DNA Gel Electrophoretic

After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None