RNA

Part:BBa_K3081116

Designed by: Xiaoying Qiao   Group: iGEM19_Peking   (2019-10-16)
Revision as of 15:03, 19 October 2019 by Xiaoying (Talk | contribs)


a kind of 20-bp sgRNA that is designed to be complementary to OriC, the genome replication origin 

The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2)  on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see BBa_K3081058


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None