Regulatory

Part:BBa_K135000

Designed by: Oliver Purcell   Group: iGEM08_BCCS-Bristol   (2008-10-20)
Revision as of 15:47, 20 October 2008 by OliverP (Talk | contribs)

pCpxR (CpxR responsive promoter)

This is an adhesion responsive promoter (specifically adhesion to hydrophobic surfaces), and can be used to upregulate downstream gene expression in response to adhesion to such surfaces. It contains the binding site for CpxR-P (phosphorylated CpxR). CpxR is one member of the two-component Cpx response to envelope stress. Briefly, envelope stress causes CpxA, an inner-membrane histidine Kinase to Phosphorylate CpxR, allowing it to bind to CpxR-P responsive regions and regulate downstream target genes. See 'Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway', Karen Otto and Thomas J. Silhavy, PNAS, Vol. 99., No, 4., 2287-2292. 2002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators1