Coding

Part:BBa_K2929001:Experience

Designed by: James Hammond   Group: iGEM19_St_Andrews   (2019-10-10)
Revision as of 14:59, 19 October 2019 by Mic3 (Talk | contribs)

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Applications of BBa_K2929001

iGEM 2019 St Andrews - Part Development

The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.

Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.


User Reviews

UNIQ1a18f016221ae652-partinfo-00000001-QINU UNIQ1a18f016221ae652-partinfo-00000002-QINU