Reporter

Part:BBa_K135001

Designed by: Oliver Purcell   Group: iGEM08_BCCS-Bristol   (2008-10-20)
Revision as of 15:38, 20 October 2008 by OliverP (Talk | contribs)

pCpxR-BBa_E0840 fusion

This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing.

This part can be used as a visual assay for adhesion to hydrophobic surfaces in E.Coli. See part BBa_K135000 for an explanation of how this works and a paper reference.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727


[edit]
Categories
Parameters
None