Regulatory

Part:BBa_K135000:Experience

Designed by: Oliver Purcell   Group: iGEM08_BCCS-Bristol   (2008-10-20)
Revision as of 14:14, 20 October 2008 by OliverP (Talk | contribs) (→‎Applications of BBa_K135000)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K135000

We have used this part, fused upstream of the GFP from BBa_E0840, and have shown GFP activation in repsonse to adhesion on glass slides. The conclusion that this is activated in response to adhesion follows from Ottos and Silhavys work (see main page description for reference). Filamentous bacteria fluoresced significantly, while it was hard to ascertain whether smaller bacteria were fluorescing. Due to time constraints, a better characterisation of the efficiency of the promoter could not be performed.

User Reviews

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