RNA

Part:BBa_K3081109

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 14:43, 19 October 2019 by Xiaoying (Talk | contribs)


a 18bp sequence targeting the R1(top strand) DnaA box on E.coli oriC

The 18 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 18bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see BBa_K3081058


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None