Composite

Part:BBa_K2981013

Designed by: Junjie Zhu   Group: iGEM19_NWU-China   (2019-10-10)
Revision as of 13:23, 19 October 2019 by WSA-JACK (Talk | contribs) (→‎Usage and Biology)


For phenylalanine-sensitive measurement pathways

For phenylalanine-sensitive measurement pathways, Phe promotes GFP expression, which is part of our measurement of Phe content.


Usage and Biology

Phe 24h fluorescence curve.
Fig.1 (A) Phe 24h fluorescence curve, (B) Linear regression curve of fluorescence intensity as a function of Phe concentration at the 24th hour


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1822
    Illegal BglII site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


[edit]
Categories
Parameters
None