Part:BBa_K2983010

Loop Alpha-A

This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow :GCTCTTCAATGAGGAGTGAGACC

Figure 1:Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine)

Blue triangle: SapI fixation site Red triangle : BsaI fixation site Blue circle : SapI cutting site Red circle: BasI cutting site

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 18
  Illegal SapI site found at 1