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Part:BBa_K2996700:Design

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-06)
Revision as of 12:32, 19 October 2019 by Pedestal (Talk | contribs)

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Flexible Linker 4 that separates RpoA and dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Highly-repeating sequence is difficult to deal with during molecular cloning and overlap extension PCR during two trials both end up in failure. So, we optimized FL4 sequence ourselves, with a simple Python program that randomly select codons for FL4.



Source

None.


References

[1]. Arai R, Ueda H, Kitayama A, etc. Design of the linkers which effectively separate domains of a bifunctional fusion protein[J]. Protein Engineering, 2001, 14(8): 529-532. [2]. Chen X, Zaro J L, Shen W C. Fusion protein linkers: property, design and functionality[J]. Advanced drug delivery reviews, 2013, 65(10): 1357-69.