Coding
T7pol

Part:BBa_K145001

Designed by: Jonas Demeulemeester and Benjamien Moeyaert   Group: iGEM08_KULeuven   (2008-07-08)
Revision as of 12:26, 19 October 2019 by Lenti (Talk | contribs)

T7 RNA polymerase

This RNA polymerase will start transcription from a T7 promoter (such as BBa_I712074).

Usage and Biology

BOKU-Vienna 2019 - Improvement

Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under BBa_K3015006

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None