Regulatory

Part:BBa_K2967011

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-09-28)
Revision as of 07:35, 19 October 2019 by Wangrui (Talk | contribs)


The inducible promoter, PhrpL

It is the sequence of inducible promoter PhrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter PhrpL. It functions like a AND logic gate to open an gene circuit. The hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None