Coding
Part:BBa_K3195004:Design
Designed by: Shan Baihui Group: iGEM19_SEU-Nanjing-China (2019-10-14)
Trx-His-EK tagged subtilisin-like protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 447
Illegal XhoI site found at 1645 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 889
Illegal AgeI site found at 1120 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 967
Design Notes
It is the coding sequence of subtilisin-like protease found in Branchiostoma. We add 6 his tag at its tail, besides we optimized its condons for better prokaryotic expression.
Source
subtilisin-like protease gene from Branchiostoma belcheri tsingtauense
References
[1]He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B http://doi.org/10.1098/rspb.2018.0438