Composite

Part:BBa_K3105672

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)
Revision as of 00:35, 19 October 2019 by JonasG (Talk | contribs)


HRP-2A-AAO

Part to co-express horseradish peroxidase (HRP) and aryl-alcohol oxidase (AAO) in Pichia pastoris. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of HRP and AAO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 1275
    Illegal BamHI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
    Illegal AgeI site found at 2422
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None