Coding

Part:BBa_K3105668

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-09-25)
Revision as of 00:04, 19 October 2019 by JonasG (Talk | contribs)


Horseradish peroxidase

Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.

HRP is often used as a conjugate with antibodies and detected by chemiluminescence.

The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidased substrate


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None