Part:BBa_K3114010
Magnesium Dechelatase Stay Green (SGR)
The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg2+) from chlorophyll, along with cofactors 2H+ (Shimoda et al. 2016)
Usage and Biology
The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg2+) from chlorophyll, along with cofactors 2H+ (Shimoda et al. 2016).
BBa_K3114010 is a golden gate compatible part which allows insertion of the SGR gene into genetic circuits to express the enzyme Magnesium Dechelatase. In creating this part, iGEM Calgary wished to allow the golden gate cloning of this plant enzyme into E.coli for other teams to use in projects involving degradation of chlorophyll. This part was designed to be golden-gate compatible based on the Mo-Clo standard (Weber et al., 2011). It is also compatible with the BioBrick RFC[10] standard.
This part is compatible with other golden gate parts created by iGEM Calgary including the following:
- DsbA signal peptide (BBa_K3114000)
- MalE signal peptide (BBa_K3114001)
- OmpA signal peptide (BBa_K3114002)
- PhoA signal peptide (BBa_K3114003)
- TorA signal peptide (BBa_K3114005)
- YcbK signal peptide (BBa_K3114004)
- Double Terminator (BBa_K3114013)
Sequences and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1508
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1883
References
Shimoda, Y., Ito, H., & Tanaka, A. (2016). Arabidopsis STAY-GREEN, Mendel’s green cotyledon gene, encodes magnesium-dechelatase. The Plant Cell, 28(9), 2147-2160.
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