Composite

Part:BBa_K3317031

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)
Revision as of 23:21, 18 October 2019 by Erick419 (Talk | contribs)


BMC shell proteins ABJKN from S. enterica LT2

Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung The plasmid contains the proteins pduA (BBa_K3317008) pduB(BBa_K3317009) pduJ (BBa_K3317010) pduK (BBa_K3317011) and pduN (BBa_K3317012)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 41
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1761
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 1388
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1691
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None