Coding

Part:BBa_K3165012:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-18)
Revision as of 22:52, 18 October 2019 by Registry (Talk | contribs)

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i^2mCherry (Leu)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed by mutations at the 46th and 48th nucleotide bases, converting the start codon (ATG) to CTT, coding for Leucine. The part was codon optimised for Escherichia coli.


Source

The sequence was derived from the existing BioBrick (BBa_J18932 : coding sequence for mCherry).

References