Composite

Part:BBa_K137079

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-19)
Revision as of 17:46, 17 October 2008 by Jkm (Talk | contribs)

AHL inducible expression of KatG

This part produces an E. coli catalase (katG) tagged with an LAA degradation tag upon induction with an acylhomoserine lactone.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/MIC_Assay minimum inhibitory concentration (MIC) assay], K137079 in pSB1A2 in SN0037 (ΔkatGΔkatE) was able to increase the MIC of SN0037 as shown below:

Strain Plasmid Highest H2O2 concentration survived (mM) Lowest H2O2 concentration inhibited (mM)
DH10B Empty
0.8
1.6
JI377 Empty
0.0
0.2
JI377 K137079, -AHL
0.3
1.0
JI377 K137079, +AHL
3.0
10.0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1316
    Illegal BglII site found at 2251
    Illegal BglII site found at 2378
    Illegal BamHI site found at 2168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


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Categories
Parameters
None