Coding

Part:BBa_K2951006

Designed by: YU-RU LIN   Group: iGEM19_CSMU_Taiwan   (2019-09-23)
Revision as of 20:04, 18 October 2019 by Yuruim (Talk | contribs) (→‎Usage and Biology)

LysRS fusion protein

Usage and Biology

This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI(NC000913.3). LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.

Characterization

This part is part of the composite part: BBa_K2951008 and further characterized.

References

1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None