Composite

Part:BBa_K3086009

Designed by: Nikila Ojili   Group: iGEM19_Florida   (2019-10-18)
Revision as of 20:00, 18 October 2019 by Registry (Talk | contribs)

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SCRIBE (RpoB) System

This is our validated part. SCRIBE( Synthetic Cellular Recorders Integrating Biological Events) is a tool that utilizes modified retrons that have been transformed into bacterial cells to produce single-stranded DNA in response to a certain stimulus. The ssDNA is incorporated into the genome using the replication system of the bacteria. This technique mutates the bacteria to have rifampicin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1282
    Illegal XhoI site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1877
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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