Composite
Part:BBa_K3086009
Designed by: Nikila Ojili Group: iGEM19_Florida (2019-10-18)
SCRIBE (RpoB) System
This is our validated part. SCRIBE( Synthetic Cellular Recorders Integrating Biological Events) is a tool that utilizes modified retrons that have been transformed into bacterial cells to produce single-stranded DNA in response to a certain stimulus. The ssDNA is incorporated into the genome using the replication system of the bacteria. This technique mutates the bacteria to have rifampicin resistance.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1877
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1877
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1282
Illegal XhoI site found at 516 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1877
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1877
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |