Coding
YFP B5-B5

Part:BBa_K2984020

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-15)
Revision as of 19:46, 18 October 2019 by Chlamy Dima (Talk | contribs)


YFP - Yellow fluorescent protein for detection B5-B5

This part is a part of the Chlamy-HUB-Collection. Yellow fluorescent protein mVenus for in vivo detection of proteins in C. reinhardtii or its surroundings. The YFP mVenus has an excitation peak at 515 nm and an emission peak at 528 nm (Kremers et al. 2006).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Kremers, G. J., Goedhart, J., van Munster, E. B., & Gadella, T. W. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570-6580.
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Categories
Parameters
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